Run the Minilys at 5000 rpm, 20 sec or 5000 rpm, 40s.
Snap-frozen in N2 or not.
Triplicate by condition.
Total RNA was extracted directly from the homogenized samples using the TRI Reagent protocol before being checked for quality and quantity.
Mean concentration of total RNA from 4 extracted pool samples snap frozen before TRI Reagent addition or not and homogenization with Minilys at 5000 rpm 20s or 40s. The corresponding mean absorption ratio (260/280) are also plotted.