Instrument: Minilys homogenizer
Sample: Cupressus sempervirens pollens
Analysis was performed by Microscopic observation (X40) / 1-D (SDSPAGE) immunoblotting.
The downstream analysis (results not shown) show that Minilys dry milling is very efficient to extract quickly high concentration of proteins. However, the protein extracted by dry milling were different from the protein extracted by overnight incubation in aqueous media (PBS).