Protein extraction from cypress pollen
Instrument: Minilys homogenizer
Sample: Cupressus sempervirens pollens
Protocol:
- Precellys kits: 03961-1-003 (1.4 mm ceramic beads).
- Sample: 100 mg of Cupressus sempervirens pollens.
- Run the Minilys at 5000 rpm, 1x30s and 3x30sec (15s break).
Results:
Analysis was performed by Microscopic observation (X40) / 1-D (SDSPAGE) immunoblotting.
The downstream analysis (results not shown) show that Minilys dry milling is very efficient to extract quickly high concentration of proteins. However, the protein extracted by dry milling were different from the protein extracted by overnight incubation in aqueous media (PBS).
