Run the samples in the Bullet Blender at speed 8 for 5 minutes.
Remove tubes from the instrument and visually inspect the samples. If homogenization is unsatisfactory, run for another two minutes at speed 10.
Reference: This protocol is adapted from: Rauceo JM, Blankenship JR, Fanning S, Hamaker JJ, Deneault JS, Smith FJ, Nantel A, Mitchell AP. Regulation of the Candida albicans cell wall damage response by transcription factor Sko1 and PAS kinase Psk1. Mol Biol Cell. 2008 Jul;19(7):2741-51.