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DNA extraction from Eois moths

Instrument: Precellys® 24

Sample: Legs and/or head/thorax of single moths

Protocol:

  • Precellys® kit: 03961-1-003 (1.4mm ceramic beads).
  • Add 1 to 6 legs and/or head/thorax of single moths.
  • Run the Precellys at 5000 rpm, 2x20 sec.
  • Extract DNA with method of choice (commercial extraction kit or phenol/chloroform protocol).

Results:

Extracted DNA of Eois moths homogenized with Precellys®24. High quality DNA (>10000bp) could be obtained from all extracted specimens. Tissue lysis with Precellys®24 is very efficient and time saving as subsequent proteinase digestion can be drastically shortened. Precellys homogenizers provide fast and cross contamination safe DNA extraction.

Agarose gel electrophoresis of extracted specimens Lane 1: Size marker; lanes 2-13: DNA extracted from legs.